The new birth; that work of the Holy Spirit by which the soul, previously dead in sins, is created anew in Christ unto righteousness. It is expressed in Scripture by being born again and born from above, Joh 3:3-7; becoming a new creature, 2Co 5:17; being quickened to a new life of holiness, Eph 2:1; having Christ formed in the heart, Ga 4:19; and being made partaker of the divine nature, 2Pe 1:4.
The sole author of this change is the Holy Spirit, Joh 1:12,13 3:4 Eph 2:8-10; and he effects it ordinarily by the instrumentality of gospel truth, 1Co 4:15 Jas 1:18 1Pe 1:23. In this change the moral image of God is brought back into the soul, and the principle of supreme love to our neighbor is implanted. Regeneration, producing faith, is accompanied by justification, and by actual holiness of life, or sanctification begun, and completed when the "babe in Christ" reaches in heaven "the fulness of the stature of the perfect man" in Him. In Mt 19:28, regeneration means Christâ€™s making all things new. In Tit 3:5, "the washing of regeneration" denotes the purifying work of the Spirit in the new birth.
Bible Dictionary - American Tract Society